Journal:

Article Title: Expression of CCL28 by Reed-Sternberg Cells Defines a Major Subtype of Classical Hodgkin's Disease with Frequent Infiltration of Eosinophils and/or Plasma Cells

doi:

Figure Lengend Snippet: Summary of the Primers Used for RT-PCR

Article Snippet: Chemotaxis Assay Recombinant human CXCL16 and CCL27 were purchased from R&D Systems.

Techniques:

Journal:

Article Title: Expression of CCL28 by Reed-Sternberg Cells Defines a Major Subtype of Classical Hodgkin's Disease with Frequent Infiltration of Eosinophils and/or Plasma Cells

doi:

Figure Lengend Snippet: Chemotactic response of HD-derived cell lines to CXCL16 and CCL27. Indicated HD-derived cell lines were examined for chemotactic responses to CXCL16, the ligand for CXCR6 (A), or to CCL27, the monospecific ligand for CCR10 (B), both at nM. For details, see Materials and Methods. Representative results from at least two separate assays are shown.

Article Snippet: Chemotaxis Assay Recombinant human CXCL16 and CCL27 were purchased from R&D Systems.

Techniques: Derivative Assay

Journal:

Article Title: Expression of CCL28 by Reed-Sternberg Cells Defines a Major Subtype of Classical Hodgkin's Disease with Frequent Infiltration of Eosinophils and/or Plasma Cells

doi:

Figure Lengend Snippet: Surface expression of CXCL16 and secretion of CCL28 by HD-derived cell lines. A: Six HD-derived cell lines were stained for surface CXCL16 by using goat anti-CXCL16. Isotype-matched goat IgG served as negative control. For details, see Materials and Methods. Representative results from at least two separate experiments are shown. Percentages of positive cells are indicated. B: Cells from indicated HD-derived cell lines were seeded in a 24-well plate at a density of 1 × 106 cells/well and cultured for 3 days. CCL28 secreted in culture supernatants was measured with enzyme-linked immunosorbent assay. All assays were done in triplicate. For details, see Materials and Methods. Representative results from two separate experiments are shown.

Article Snippet: Chemotaxis Assay Recombinant human CXCL16 and CCL27 were purchased from R&D Systems.

Techniques: Expressing, Derivative Assay, Staining, Negative Control, Cell Culture, Enzyme-linked Immunosorbent Assay

Journal: Frontiers in Immunology

Article Title: Phenotypic and Functional Plasticity of CXCR6 + Peripheral Blood NK Cells

doi: 10.3389/fimmu.2021.810080

Figure Lengend Snippet: Mass cytometry immunophenotyping detects subsets of CXCR6 + NK cells with diverse expression patterns, distinct from that of CXCR6- NK cells. (A) Hierarchical gating strategy of cultured PBMC derived CXCR6 + and CXCR6 - NK cell cultures. CXCR6 + and CXCR6 - NK cells were single cell sorted from PBMCs ( n=8 donors) and cultured with IL-15 and CXCL16 feeder cells for 2-7 weeks. Cultured cells were stained for 32 cell surface and intracellular targets and analyzed by mass cytometry. (B) t-Distributed Stochastic Neighbor Embedding (t-SNE) dimensionality reduction and Rphenograph clustering of CXCR6 + and CXCR6 - NK cells. (C) Expression patterns of selected markers.

Article Snippet: Because CXCR6 + NK cells depend on CXCL16 expression for survival in the liver sinusoidal epithelium ( , ), we transduced a B lymphoblastoid cell line (RPMI-8866) with lentiviral particles (pLenti-C-mGFP, Origene) encoding either CXCL16 transcript variant 1 (TV1, NM_022059) or 2 (TV2, NM_001100812), thereby generating B cell lines stably expressing the ligand for CXCR6.

Techniques: Mass Cytometry, Expressing, Cell Culture, Derivative Assay, Staining

Journal: Endocrine-Related Cancer

Article Title: CXCL16 signaling mediated macrophage effects on tumor invasion of papillary thyroid carcinoma

doi: 10.1530/erc-15-0196

Figure Lengend Snippet: Figure 2. Enhanced CXCL16 expressions in PTC tumor microenvironment. (A) CXCL16 levels were measured

Article Snippet: Reagents 72 Recombinant human CXCL16, anti-human CXCL16 neutralizing antibody, and human CXCL16 73 Quantikine ELISA Kit were purchased from R&D system (Minneapolis, MN, USA).

Techniques:

Journal: Endocrine-Related Cancer

Article Title: CXCL16 signaling mediated macrophage effects on tumor invasion of papillary thyroid carcinoma

doi: 10.1530/erc-15-0196

Figure Lengend Snippet: Figure 4. CXCL16 mediated macrophage polarization in PTC tumor microenvironments. (A) Primary monocytes were treated with 50% CMs from H tori, BHP10-3, and KTC-1. CMs of BHP10-3 and KTC-1 groups were treated with anti-CXCL16 antibodies (100 ug/ml), simultaneously. mRNA expressions of CD163,

Article Snippet: Reagents 72 Recombinant human CXCL16, anti-human CXCL16 neutralizing antibody, and human CXCL16 73 Quantikine ELISA Kit were purchased from R&D system (Minneapolis, MN, USA).

Techniques:

Journal: Endocrine-Related Cancer

Article Title: CXCL16 signaling mediated macrophage effects on tumor invasion of papillary thyroid carcinoma

doi: 10.1530/erc-15-0196

Figure Lengend Snippet: Figure 5. Expression of CXCR6 or CXCL16 in PTC tissues. Immunohistochemical staining was done in the PTC tissue microarray with anti-CXCR6 or anti-CXCL16 antibodies (n=143). Representative images of (A) cancer-

Article Snippet: Reagents 72 Recombinant human CXCL16, anti-human CXCL16 neutralizing antibody, and human CXCL16 73 Quantikine ELISA Kit were purchased from R&D system (Minneapolis, MN, USA).

Techniques: Expressing, Immunohistochemical staining, Staining, Microarray